These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources.
Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. In the area of molecular diagnostics, PCR has allowed target detection to be performed with unprecedented sensitivity and ease.
It also briefs the techniques which can be used in diagnosis of different diseases. It is hoped that the step-by-step protocols and the explanatory notes will help readers to harness the power of these techniques in their laboratories.
In short the aim of this handbook is to provide a meaningful and comprehensive picture of PCR and their types. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter.
Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing mutation detection, gene expression, etc.
All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor.
The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis.
Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management.
Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field. Skip to main content Skip to table of contents.
Advertisement Hide. This service is more advanced with JavaScript available. Authors view affiliations Gerrit J. Viljoen Louis H. Nel John R. Gives a comprehensive account of the practical aspects of PCR Strong consideration is given to ensure its optimal use in a laboratory environment. Front Matter Pages i Pages Limitations and Troubleshooting. The PCR Laboratory.
Back Matter Pages In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction PCR.
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